Fluorescent PCR Instrument

Fluorescent PCR instruments are primarily used to perform real-time quantitative PCR experiments. By stimulating and collecting fluorescence signals, they enable real-time monitoring of the experimental process. Experimental data is plotted into fluorescence curves and displayed in real time on the instrument interface. The instruments then perform data fitting and analysis, ultimately generating experimental reports in PDF format.

It enables rapid and precise qualitative and quantitative analysis of target nucleic acids present in biological samples (such as blood or bodily fluids) extracted from other animals. Additionally, it supports analyses of special samples including standard curve fitting, melting curve analysis, and genotyping.

Principle of Fluorescent PCR Instruments: Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA segments. It functions as an in vitro DNA amplification method, comprising a temperature cycle consisting of high-temperature denaturation, low-temperature annealing, and optimal-temperature extension. This process enables rapid amplification of target genes with high specificity, sensitivity, ease of operation, and time efficiency, representing a major innovation in gene amplification technology. PCR can amplify minute amounts of target DNA millions of times, significantly enhancing the analysis and detection capabilities of DNA molecules.

Real-time quantitative PCR technology builds upon conventional PCR by incorporating fluorescent dyes or fluorescently labeled probes. Throughout the PCR reaction, changes in fluorescent signals enable real-time monitoring of the entire process. This method employs fluorescent chemicals to track the total product yield after each PCR cycle, allowing quantitative analysis of specific DNA sequences within the sample using either internal or external controls.

Applications include: fundamental scientific research, pathogen detection, animal disease surveillance, meat adulteration detection, genetically modified organism (GMO) testing, food hygiene inspection, drug development and rational drug use, gene expression analysis, and water quality monitoring.

• Real-time dynamic monitoring: Visualize the entire experimental process with automatic generation of fluorescence amplification curves.

• Multi-dimensional analysis: Supports professional analyses including standard curves, melting curves, and genotyping to meet complex detection requirements.

• Features high specificity, sensitivity, ease of operation, and time efficiency.

• High-definition touchscreen operation requires no training for effortless use.

• Efficient report generation with automatic PDF report creation.